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← Microbiology / 2nd Year / MBBS

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Autoclave

Identify a steam steriliser and explain validation and load-dependent performance.

REFERENCE GALLERY1 / 1 reference
autoclave — reference view
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Chamber door

The front closure of the right-hand steriliser chamber; it remains secured during operation.

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autoclave — reference view

Image: The original uploader was Scharks at English Wikipedia. · Source / original · CC BY 2.5

Resized and converted to WebP; original colours retained.

Autoclave

Identify and explain

Moist heat under controlled conditions inactivates microorganisms; a validated cycle depends on the load and equipment.

  • Sealed pressure chamber
  • Door or lid with locking mechanism
  • Operating controls and status indicators
1

Identify

Distinguish steam sterilisation from dry-heat equipment.

2

Validate

Use the approved cycle and monitoring for the specific load.

3

Document

Review process records and indicator results; never assume performance from heat alone.

At the viva table

Try answering aloud, then open the suggested answer. Examiner questions vary.

01 / Is pressure alone the sterilising agent?

No. It supports the steam conditions needed for effective moist-heat treatment.

02 / Can every load use the same cycle?

No. Packaging, volume, material and equipment affect validation.

03 / Does indicator colour alone prove every item sterile?

No. Indicators are interpreted within a validated quality system.

04 / When can the chamber be opened?

Only when the equipment’s approved safety conditions and procedure permit it.

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