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Volumetric pipette — reference view
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Calibration mark

The meniscus is aligned with the calibration line for the specified transfer volume.

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Volumetric pipette — reference view

Image: Zelda F. Scott · Source / original · CC BY-SA 3.0

Resized and converted to WebP; original colours retained.

Volumetric pipette

Liquid measurement

Volumetric pipette

Transfer pipette · bulb pipette

THE FIRST VIVA QUESTION

What is its use?

Transfers a fixed calibrated volume for accurate solution preparation.

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01 / IDENTIFICATION

How to recognise it.

  1. Central bulb
  2. Single calibration mark on the stem
  3. Tapered delivery tip
Tell the difference.

A volumetric pipette delivers one specified volume; a graduated pipette can deliver a range of volumes.

02 / NUMBERED PARTS

Point them out.

Choose a part to bring its numbered marker into view.

03 / WORKING PRINCIPLE

The science behind the reading.

The meniscus is aligned with a calibration mark; the pipette delivers its specified volume under stated conditions.

04 / VIVA PRACTICE

Think first. Then reveal.

Explain your answer aloud before opening it.

01Why use a pipette filler?
SUGGESTED ANSWER

It permits controlled aspiration without mouth pipetting.

02Should the final drop always be blown out?
SUGGESTED ANSWER

No. Delivery depends on the pipette’s calibration and marking; many transfer pipettes retain a final drop.

03Where is the meniscus read?
SUGGESTED ANSWER

At eye level; for clear aqueous liquids, the bottom of the concave meniscus is normally aligned with the mark.

04Why rinse with the solution when appropriate?
SUGGESTED ANSWER

Residual water would dilute the solution being measured.

Read the references.

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